Plasma‐mediated enhancement of enzyme secretion in Aspergillus oryzae
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چکیده
منابع مشابه
Aminoacylase from Aspergillus oryzae. Comparison with the pig kidney enzyme.
Aminoacylase (EC 3.5.1.14) from Aspergillus oryzae was purified from a commercially available crude material by heat treatment, precipitation by polyethyleneimine and ammoniumsulfate, gel chromatography and preparative disc-gel-electrophoresis. The purified product was homogenous as judged by polyacrylamide gel electrophoresis. SDS-gel electrophoresis, polyacrylamide-gel-gradient electrohoresis...
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Although the production of proteolytic enzymes by different strains of Aspergillus oryzae has been investigated in submerged culture (5, 15), in static liquid culture (3, 11), and on solid substratum (8-10), there appear to be few reports pertaining to the comparative evaluation of these different methods on the elaboration of proteinases by specific strains of A. oryzae. K. Mogi (13) compared ...
متن کاملTrehalase in conidia of Aspergillus oryzae.
Horikoshi, Koki (The Institute of Physical and Chemical Research, Bunkyo-ku, Tokyo, Japan), and Yonosuke Ikeda. Trehalase in conidia of Aspergillus oryzae. J. Bacteriol. 91:1883-1887. 1966.-Trehalases (soluble trehalase and coat-bound trehalase) were found in the conidia of Aspergillus oryzae, and the total activity of the trehalases increased during the germination process. The soluble trehala...
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To produce Aspergillus oryzae alkaline protease (Alp) in an osmophilic yeast Zygosaccharomyces rouxii, we constructed an expression plasmid consisting of the Z. rouxii glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter, the prepro-Alp cDNA of A. oryzae, the whole sequence of Z. rouxii plasmid pSR1, and the G418 resistant gene. The resulting plasmid, when introduced into Z. rouxii cells, ...
متن کاملDeletion of creB in Aspergillus oryzae increases secreted hydrolytic enzyme activity.
Aspergillus oryzae has been used in the food and beverage industry for centuries, and industrial strains have been produced by multiple rounds of selection. Targeted gene deletion technology is particularly useful for strain improvement in such strains, particularly when they do not have a well-characterized meiotic cycle. Phenotypes of an Aspergillus nidulans strain null for the CreB deubiquit...
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ژورنال
عنوان ژورنال: Microbial Biotechnology
سال: 2020
ISSN: 1751-7915,1751-7915
DOI: 10.1111/1751-7915.13696